Institute of Biochemistry


Müller IK, Winter C, Thomas C, Spaapen RM, Trowitzsch S*, Tampé R*
Structure of an MHC I–tapasin–ERp57 editing complex defines chaperone promiscuity
Nat Commun. 2022;13:5383.
* co-correspondence

Sekulovski S, Sušac S, Stelzl LS, Tampé R, Trowitzsch S
Structural basis of substrate recognition by human tRNA splicing endonuclease TSEN

Domnick A+, Winter C+, Sušac L, Hennecke L, Hensen M, Zitzmann N, Trowitzsch S, Thomas C, Tampé R
Molecular basis of MHC I quality control in the peptide loading complex
Nat Commun. 2022 Aug 10;13(1):4701
PMID: 35948544
+ shared first authors

Sekulovski S, Trowitzsch S
Transfer RNA processing - from a structural and disease perspective
Biol Chem. 2022 Jun 21;403(8-9):749-763
PMID: 35728022

Sethumadhavan S, Barth M, Spaapen RM, Schmidt C, Trowitzsch S, Tampé R
Viral immune evasins impact antigen presentation by allele-specific trapping of MHC I at the peptide-loading complex
Sci Rep. 2022 Jan 27;12(1):1516
PMID: 35087068

Sekulovski S+, Devant P+, Panizza S+, Gogakos T, Pitiriciu A, Heitmeier K, Ramsay EP, Barth M, Schmidt C, Weitzer S, Tuschl T, Baas F, Martinez J*, Trowitzsch S*
Assembly defects of the human tRNA splicing endonuclease contribute to impaired pre-tRNA processing in pontocerebellar hypoplasia
Nat Commun. 2021 Sep 28;12(1):5610
PMID: 34584079
+ shared first authors
* co-correspondence

Trowitzsch S*, Tampé R*
Multifunctional Chaperone and Quality Control Complexes in Adaptive Immunity.
Annu Rev Biophys. 2020 May 6;49:135-161.
PMID: 32004089
* co-correspondence

Trowitzsch S*, Tampé R*
ABC Transporters in Dynamic Macromolecular Assemblies.
J Mol Biol. 2018 Oct 26;430(22):4481-4495.

PMID: 30089236
* co-correspondence

Blees A+, Januliene D+, Hofmann T, Koller N, Schmidt C, Trowitzsch S*, Moeller A*, Tampé R*
Structure of the human MHC-I peptide-loading complex.
Nature. 2017 Nov 23;551(7681):525-528.

PMID: 29107940
+ shared first authors
* co-correspondence

Heuer A, Gerovac M, Schmidt C, Trowitzsch S, Preis A, Kötter P, Berninghausen O, Becker T, Beckmann R, Tampé R
Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation.
Nat Struct Mol Biol. 2017 May;24(5):453-460.

PMID: 28368393

Herbring V, Bäucker A, Trowitzsch S, Tampé R
A dual inhibition mechanism of herpesviral ICP47 arresting a conformationally thermostable TAP complex.
Sci Rep. 2016 Nov 15;6:36907.

PMID: 27845362

Gupta K, Sari-Ak D, Haffke M, Trowitzsch S, Berger I
Zooming in on Transcription Preinitiation.
J Mol Biol. 2016 Jun 19;428(12):2581-2591.

PMID: 27067110

Blees A, Reichel K, Trowitzsch S, Fisette O, Bock C, Abele R, Hummer G, Schäfer LV, Tampé R
Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch.
Sci Rep. 2015 Nov 27;5:17341.

PMID: 26611325

Riss A, Scheer E, Joint M, Trowitzsch S, Berger I, Tora L
Subunits of ADA-two-A-containing (ATAC) or Spt-Ada-Gcn5-acetyltrasferase (SAGA) Coactivator Complexes Enhance the Acetyltransferase Activity of GCN5.
J Biol Chem. 2015 Nov 27;290(48):28997-9009.

PMID: 26468280

Trowitzsch S, Tampé R
Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes.
Methods Enzymol. 2015;557:3-26.

PMID: 25950957

Nguyen-Huynh NT, Sharov G, Potel C, Fichter P, Trowitzsch S, Berger I, Lamour V, Schultz P, Potier N, Leize-Wagner E
Chemical cross-linking and mass spectrometry to determine the subunit interaction network in a recombinant human SAGA HAT subcomplex.
Protein Sci. 2015 Aug;24(8):1232-46.

PMID: 25753033

Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I
Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.
Nat Commun. 2015 Jan 14;6:6011.

PMID: 25586196

Wysoczański P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Lührmann R, Becker S, Zweckstetter M
Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.
Nat Struct Mol Biol. 2014 Oct;21(10):911-8.

PMID: 25218446

Kandiah E, Trowitzsch S, Gupta K, Haffke M, Berger I
More pieces to the puzzle: recent structural insights into class II transcription initiation.
Curr Opin Struct Biol. 2014 Feb;24:91-7.

PMID: 24440461

Nie Y, Bellon-Echeverria I, Trowitzsch S, Bieniossek C, Berger I
Multiprotein complex production in insect cells by using polyproteins.
Methods Mol Biol. 2014;1091:131-41.

PMID: 24203328

Trowitzsch S, Palmberger D, Fitzgerald D, Takagi Y, Berger I
MultiBac complexomics.
Expert Rev Proteomics. 2012 Aug;9(4):363-73.

PMID: 22967074

Trowitzsch S, Klumpp M, Thoma R, Carralot JP, Berger I
Light it up: highly efficient multigene delivery in mammalian cells.
Bioessays. 2011 Dec;33(12):946-55.

PMID: 22002169

Vijayachandran LS, Viola C, Garzoni F, Trowitzsch S, Bieniossek C, Chaillet M, Schaffitzel C, Busso D, Romier C, Poterszman A, Richmond TJ, Berger I
Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells.
J Struct Biol. 2011 Aug;175(2):198-208.

PMID: 21419851

Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, Trowitzsch S, Will CL, Lührmann R, Söll D, Martinez J
HSPC117 is the essential subunit of a human tRNA splicing ligase complex.
Science. 2011 Feb 11;331(6018):760-4.

PMID: 21311021

Weber G, Trowitzsch S, Kastner B, Lührmann R, Wahl MC
Functional organization of the Sm core in the crystal structure of human U1 snRNP.
EMBO J. 2010 Dec 15;29(24):4172-84.

PMID: 21113136

Nie Y, Viola C, Bieniossek C, Trowitzsch S, Vijay-Achandran LS, Chaillet M, Garzoni F, Berger I
Getting a grip on complexes.
Curr Genomics. 2009 Dec;10(8):558-72.

PMID: 20514218

Strzelecka M, Trowitzsch S, Weber G, Lührmann R, Oates AC, Neugebauer KM
Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis.
Nat Struct Mol Biol. 2010 Apr;17(4):403-9.

PMID: 20357773

Brakemann T, Weber G, Andresen M, Groenhof G, Stiel AC, Trowitzsch S, Eggeling C, Grubmüller H, Hell SW, Wahl MC, Jakobs S
Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron.
J Biol Chem. 2010 May 7;285(19):14603-9.

PMID: 20236929

Trowitzsch S, Bieniossek C, Nie Y, Garzoni F, Berger I
New baculovirus expression tools for recombinant protein complex production.
J Struct Biol. 2010 Oct;172(1):45-54.

PMID: 20178849

Trowitzsch S, Weber G, Lührmann R, Wahl MC
Crystal structure of the Pml1p subunit of the yeast precursor mRNA retention and splicing complex.
J Mol Biol. 2009 Jan 16;385(2):531-41.

PMID: 19010333

Trowitzsch S, Weber G, Lührmann R, Wahl MC
An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex.
J Biol Chem. 2008 Nov 21;283(47):32317-27.
PMID: 18809678

Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S
Structural basis for reversible photoswitching in Dronpa.
Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9.

PMID: 17646653

Stiel AC+, Trowitzsch S+, Weber G+, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC
1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.
Biochem J. 2007 Feb 15;402(1):35-42.

PMID: 17117927
+ shared first authors

Savarese E, Chae OW, Trowitzsch S, Weber G, Kastner B, Akira S, Wagner H, Schmid RM, Bauer S, Krug A
U1 small nuclear ribonucleoprotein immune complexes induce type I interferon in plasmacytoid dendritic cells through TLR7.
Blood. 2006 Apr 15;107(8):3229-34.

PMID: 16368889

Andresen M, Wahl MC, Stiel AC, Gräter F, Schäfer LV, Trowitzsch S, Weber G, Eggeling C, Grubmüller H, Hell SW, Jakobs S
Structure and mechanism of the reversible photoswitch of a fluorescent protein.
Proc Natl Acad Sci USA. 2005 Sep 13;102(37):13070-4.

PMID: 16135569


Dr. Simon Trowitzsch

Group leader
Biocenter N210/1.07
phone  +49 (0)69 798 29273
email  trowitzsch(at)

twitter @TrowitzschLab