Institute of Biochemistry

Krüger H, Asido M, Wachtveitl J, Tampé R, Wieneke R
Sensitizer-enhanced two-photon patterning of biomolecules in photoinstructive hydrogels.
Commun Mater 3, 9 (2022)

Sánchez MF, Dietz MS, Müller U, Weghuber J, Gatterdam K, Wieneke R, Heilemann M, Lanzerstorfer P, Tampé R
Dynamic in situ confinement triggers ligand-free neuropeptide receptor signaling.
Nano Lett. 2022, 22, 20, 8363-8371

Zhou R, Canena D, Sikora M, Klausberger M, Seferovic H, Mehdipour AR, Hain L, Laurent E, Monteil V, Wirnsberger G, Wieneke R, Tampé R, Kienzl NF, Mach L, Mirazimi A, Oh YJ, Penninger JM, Hummer G, Hinterdorfer P
Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level - MD simulations dataset.
10.5281/zenodo.7401939 (2022)

Brunnberg J, Herbring V, Günther Castillo E, Krüger H, Wieneke R, Tampé R
Light control of the peptide-loading complex synchronizes antigen translocation and MHC I trafficking.
Commun Biol 4, 430 (2021)

Sánchez MF, Els Heindl S, Beck Sickinger A, Wieneke R, Tampé R
Photoinduced receptor confinement drives ligand-independent GPCR signaling.
Science 371, eabb7657 (2021)

Khoo KK, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen MH, Chua HC, Wulf M, Tampé R, Pless SA
Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing.
Nat Commun 11, 2284 (2020)

Liamas E, Black RA, Mulheran PA, Tampé R, Wieneke R, Thomas ORT, Zhang ZJ
Probing fibronectin adsorption on chemically defined surfaces by means of single molecule force microscopy.
Sci Rep 10, 15662 (2020)

Baldering TN, Dietz MS, Gatterdam K, Karathanasis C, Wieneke R, Tampé R, Heilemann M.
Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.
Mol Biol Cell. 2019 Apr 10:mbcE18100661. doi: 10.1091/mbc.E18-10-0661. [Epub ahead of print]

Wieneke R, Tampé R.
Multivalent Chelators for In Vivo Protein Labeling.
Angew Chem Int Ed Engl. 2019 Mar 27. doi: 10.1002/anie.201811293. [Epub ahead of print]

Braner M, Koller N, Knauer J, Herbring V, Hank S, Wieneke R, Tampé R*. 
Optical control of the antigen translocation by synthetic photo-conditional viral inhibitors.
Chemical Science, 2019, 10, 2001 - 2005
                 *shared corresponding authorship 

Klein A, Hank S, Raulf A, Joest EF, Tissen F, Heilemann M, Wieneke R*, Tampé R*.
Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies
Chemical Science, 2018, DOI: 10.1039/C8SC02910E
                 *shared corresponding authorship           

Glembockyte V, Wieneke R, Gatterdam K, Gidi Y, Tampé R, Cosa G.
Tris -N-Nitrilotriacetic Acid Fluorophore as a Self-Healing Dye for Single-Molecule Fluorescence Imaging.
J Am Chem Soc. 2018 Aug 23. doi: 10.1021/jacs.8b04681.

Brzozowska E, Leśniewski A, Sęk S, Wieneke R, Tampé R, Górska S, Jönsson-Niedziółka M, Niedziółka-Jönsson J.
Interactions of bacteriophage T4 adhesin with selected lipopolysaccharides studied using atomic force microscopy.
Sci Rep. 2018 Jul 19;8(1):10935. doi: 10.1038/s41598-018-29383-w.

Venkataramani V, Kardorff M, Herrmannsdörfer F, Wieneke R, Klein A, Tampé R, Heilemann M Kuner T.
Enhanced labeling density and whole-cell 3D dSTORM imaging by repetitive labeling of target proteins.
Sci Rep. 2018 Apr 3;8(1):5507. doi: 10.1038/s41598-018-23818-0.

Bartelt SM , Chervyachkova E , Steinkühler J , Ricken J , Wieneke R , Tampé R , Dimova R , Wegner SV.
Dynamic blue light-switchable protein patterns on giant unilamellar vesicles.
Chem Commun (Camb). 2018 Jan 23;54(8):948-951. doi: 10.1039/c7cc08758f.

Braner M, Wieneke R and Tampé R.
Nanomolar Affinity Protein Trans-Splicing Monitored in Real-Time
by Fluorophore-Quencher Pairs

Chem. Commun., 2017, 545-8. DOI: 10.1039/C6CC08862G.

Robb NC, Te Velthuis AJ, Wieneke R, Tampé R, Cordes T, Fodor E, Kapanidis AN.
Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA.
Nucleic Acids Res. 2016 Sep 30. pii: gkw884.

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, García RR, Angevin M, Heichette C, Wieneke R, Tampé R, Duchesne L, Akhmanova A, Steinmetz MO, Chrétien D.
EB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Nat Cell Biol. 2016 Oct;18(10):1102-8. doi: 10.1038/ncb3412.[Epub ahead of print]

Kollmannsperger A, Sharei A, Raulf A, Heilemann M, Langer R, Jensen KF, Wieneke R*, Tampé R*.
Live-cell protein labelling with nanometre precision by cell squeezing.
Nat Commun. 2016 Jan 29;7:10372. doi: 10.1038/ncomms10372.
                 *shared corresponding authorship
                  highlighted on the MIT News homepage

Braner M, Kollmannsperger A, Wieneke R and Tampé R.
‘Traceless’ tracing of proteins – high-affinity trans-splicing directed by a minimal interaction pair
Chem. Sci., 2016, 7, 2646-2652 DOI: 10.1039/C5SC02936H

Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin SR, Tampé R, Kobilka BK, Müller DJ.
Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
Nat Commun. 2015 Nov 12;6:8857. doi: 10.1038/ncomms9857.

Fleischmann G, Fisette O, Thomas C, Wieneke R, Tumulka F, Schneeweiss C, Springer S, Schäfer LV, Tampé R.
Mechanistic Basis for Epitope Proofreading in the Peptide-Loading Complex.
J Immunol. 2015 Sep 28. pii: 1501515.
                  featured by the ‘In This Issue’ section to be among the top 10% of
                  articles published in the journal

Wieneke R, Raulf A, Kollmannsperger A, Heilemann M, Tampé R.
SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.
Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10216-9. doi: 10.1002/anie.201503215. Epub 2015 Jul 15.
                  highligted and selected for the Angew. Chem. front page
                  read more

Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP.
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm. 2015 Jul 10. pii: S0939-6411(15)00290-8. doi: 10.1016/j.ejpb.2015.07.002. [Epub ahead of print]

Lin J, Eggensperger S, Hank S, Wycisk A, Wieneke R, Mayerhofer PU, Tampé R
A negative feedbackmodulator of antigen processing evolved from a frameshift in the cowpox virus genome.
PLoS Pathog. 2014 Dec 11,DOI: 10.1371/journal.ppat.1004554

Wieneke R, Laboria N , Rajan M, Kollmannsperger A, Natale F, Cardoso MC , Tampé R.
Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes
J. Am. Chem. Soc., 2014, 136 (40), pp 13975–13978 DOI: 10.1021/ja5063357.
                  awarded as outstanding publication in the SPP 1623

Labòria N, Wieneke R, Tampé R.
Control of nanomolar interaction and in situ assembly of proteins in four dimensions by light.
Angew Chem Int Ed Engl. 2013 Jan 14;52(3):848-53.

Wei R, Gatterdam V, Wieneke R, Tampé R, Rant U.
Stochastic sensing of proteins with receptor-modified solid-state nanopores.
Nat Nanotechnol. 2012 Mar 11;7(4):257-63.
News & Views Review:
                  Gu LQ, Ritzo B, Wang Y.
                  Biosensing: When less is more in a nanopore.
                  Nat Nanotechnol. 2012 Apr 5;7(4):212-3.
Wycisk AI, Lin J, Loch S, Hobohm K, Funke J, Wieneke R, Koch J, Skach WR, Mayerhofer PU, Tampé R.
Epstein-Barr viral BNLF2a hijacks the tail-anchored protein insertion machinery to block antigen processing by the TAP complex.
J Biol Chem. 2011 286: 41402–41412.

Wieneke R, Bernecker A, Riedel R, Sumper M, Steinem C, Geyer A.
Silica precipitation with synthetic silaffin peptides.
Org Biomol Chem. 2011 Aug 7;9(15):5482-6. doi: 10.1039/c1ob05406f. Epub 2011 Jun 15.

Bernecker A, Wieneke R, Riedel R, Seibt M, Geyer A, Steinem C.
Tailored synthetic polyamines for controlled biomimetic silica formation.
J Am Chem Soc. 2010 Jan 27;132(3):1023-31. doi: 10.1021/ja9061163.
                  highlighted as one of the most important papers in the field of
                  surface chemistry in the year 2010
                  read more

Bernecker A, Ziomkowska J, Heitmüller S, Wieneke R, Geyer A, Steinem C.
Formation of silica precipitates on membrane surfaces in two and three dimensions.
Langmuir. 2010 Aug 17;26(16):13422-8. doi: 10.1021/la1021627.

Marimganti S, Wieneke R, Geyer A,Maier ME.                        
Synthesis and Conformational Analysis of Geodiamolide Analogues.
Eur. J. Org. Chem. 2007, 2779-2790.

Wieneke R, Klein S, Geyer A, Loos E.
Structural and functional characterization of galactooligosaccharides in Nostoc commune: beta-D-galactofuranosyl-(1-->6)-[beta-D-galactofuranosyl-(1-->6)]2-beta-D-1,4-anhydrogalactitol and beta-(1-->6)-galactofuranosylated homologues.
Carbohydr Res. 2007 Dec 28;342(18):2757-65. Epub 2007 Sep 14.


Dr. Ralph Wieneke
Institute of Biochemistry,
Biocenter N220/1.04
Goethe-University Frankfurt
Max-von-Laue-Str. 9
D-60438 Frankfurt/M., Germany
Phone: +49-(0)-69 - 798 29477