Institute of Biochemistry
Publications 2016

Herbring V, Bäucker A,Trowitzsch S, Tampé R
A dual inhibition mechanism of herpesviral ICP47 arresting a conformationally thermostable TAP complex
Scientific Reports 6, 36907

Kiosze-Becker K, Ori A, Gerovac M, Heuer A, Nürenberg-Goloub E, Jan Rashid U,
Becker T, Beckmann R, Beck M, Tampé R,
Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry
Nature Communications 2016/11/08/online

Robb NC, Te Velthuis AJ, Wieneke R, Tampé R, Cordes T, Fodor E, Kapanidis AN.
Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA.
Nucleic Acids Res. 2016 Sep 30. pii: gkw884.

Lehnert E, Mao J, Mehdipour AR, Hummer G, Abele R, Glaubitz C, Tampé R.
Antigenic peptide recognition on the human ABC transporter TAP resolved by DNP-enhanced solid-state NMR spectroscopy.
J Am Chem Soc. 2016 Sep 23. [Epub ahead of print]

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, García RR, Angevin M, Heichette C, Wieneke R, Tampé R, Duchesne L, Akhmanova A, Steinmetz MO, Chrétien D.
EB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Nat Cell Biol. 2016 Oct;18(10):1102-8. doi: 10.1038/ncb3412.[Epub ahead of print]

Casanas, A, Warshamanage, R, Finke, A D, Panepucci, E, Olieric, V, Noll, A, Tampe, R, Brandstetter, S, Forster, A, Mueller, M, Schulze-Briese, C, Bunk, O & Wang, M.
EIGER detector: application in macromolecular crystallography
Acta Crystallographica Section D 2016;72(9):1036-1048doi:10.1107/S2059798316012304

Posch S, Aponte-Santamaría C, Schwarzl R, Karner A, Radtke M, Gräter F, Obser T, König G, Brehm MA, Gruber HJ, Netz RR, Baldauf C, Schneppenheim R, Tampé R, Hinterdorfer P.
Single molecule force spectroscopy data and BD- and MD simulations on the blood protein von Willebrand factor.
Data Brief. 2016 Jul 21;8:1080-7. doi: 10.1016/j.dib.2016.07.031. eCollection 2016.

Michael Urban, Marc Vor der Brüggen, Robert Tampé.
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip Systemat Single Protein Resolution
J Vis Exp. 2016 Aug 16;(114). doi: 10.3791/53373.

Schmidt A, Brettschneider K, Kahle J, Orlowski A, Becker-Peters K, Stichel D, Schulze J, Braner M, Tampé R, Schwabe D, Königs C.
Neutralisation of factor VIII inhibitors by anti-idiotypes isolated from phage-displayed libraries.
Thromb Haemost. 2016 Mar 24;116(1). [Epub ahead of print]

Braner M, Kollmannsperger A, Wieneke R and Tampé R. 
‘Traceless’ tracing of proteins – high-affinity trans-splicing directed by a minimal interaction pair
Chem. Sci., 2016, 7, 2646-2652 DOI: 10.1039/C5SC02936H

Kollmannsperger A, Sharei A, Raulf A, Heilemann M, Langer R, Jensen KF, Wieneke R, Tampé R.
Live-cell protein labelling with nanometre precision by cell squeezing.
Nat Commun. 2016 Jan 29;7:10372. doi: 10.1038/ncomms10372.

Fisette O, Wingbermühle S, Tampé R, Schäfer LV.
Molecular mechanism of peptide editing in the tapasin-MHC I complex.
Sci Rep. 2016 Jan 12;6:19085. doi: 10.1038/srep19085.

Heise R, Amann PM, Ensslen S, Marquardt Y, Czaja K, Joussen S, Beer D, Abele R, Plewnia G, Tampé R, Merk HF, Hermanns HM, Baron JM.
Interferon Alpha Signalling and Its Relevance for the Upregulatory Effect of Transporter Proteins Associated with Antigen Processing (TAP) in Patients with Malignant Melanoma.
PLoS One. 2016 Jan 6;11(1):e0146325. doi: 10.1371/journal.pone.0146325. eCollection 2016.

Publications 2015

Blees A, Reichel K, Trowitzsch S, Fisette O, Bock C, Abele R, Hummer G, Schäfer LV, Tampé R.
Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch.
Sci Rep. 2015 Nov 27;5:17341. doi: 10.1038/srep17341.

Urban M, Tampé R.
Membranes on nanopores for multiplexed single-transporter

Microchim Acta, DOI 10.1007/s00604-015-1676-4

Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin SR, Tampé R, Kobilka BK, Müller DJ.
Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
Nat Commun. 2015 Nov 12;6:8857. doi: 10.1038/ncomms9857.

Fleischmann G, Fisette O, Thomas C, Wieneke R, Tumulka F, Schneeweiss C, Springer S, Schäfer LV, Tampé R.
Mechanistic Basis for Epitope Proofreading in the Peptide-Loading Complex.
J Immunol. 2015 Sep 28. pii: 1501515.

Wieneke R, Raulf A, Kollmannsperger A, Heilemann M, Tampé R.
SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.
Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10216-9. doi: 10.1002/anie.201503215. Epub 2015 Jul 15.

Strale PO, Duchesne L, Peyret G, Montel L, Nguyen T, Png E, Tampé R, Troyanovsky S, Hénon S, Ladoux B, Mège RM.
The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity.
J Cell Biol. 2015 Jul 20;210(2):333-46. doi: 10.1083/jcb.201410111.

Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP.
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm. 2015 Jul 10. pii: S0939-6411(15)00290-8. doi: 10.1016/j.ejpb.2015.07.002.

Trowitzsch S, Tampé R.
Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes.
Methods Enzymol. 2015;557:3-26. doi: 10.1016/bs.mie.2014.11.043. Epub 2015 Jan 10.

Tampé R.
Membrane Transport and Communication in Frankfurt: Speakers' Summary - Highlights.
Biol Chem. 2015 Sep 1;396(9-10):949-54. doi: 10.1515/hsz-2015-0211.

Kemmer GC, Bogh SA, Urban M, Palmgren MG, Vosch T, Schiller J, Günther Pomorski T.
Lipid-conjugated fluorescent pH sensors for monitoring pH changes in reconstituted membrane systems.
Analyst. 2015 Aug 24;140(18):6313-20. doi: 10.1039/c5an01180a.

Eggensperger S, Tampé R.
The transporter associated with antigen processing: a key player in adaptive immunity.
Biol Chem. 2015 Sep 1;396(9-10):1059-72. doi: 10.1515/hsz-2014-0320.

Zollmann T, Bock C, Graab P, Abele R.
Team work at its best - TAPL and its two domains.
Biol Chem. 2015 Feb 25. pii: /j/bchm.just-accepted/hsz-2014-0319/hsz-2014-0319.xml. doi: 10.1515/hsz-2014-0319.

Mayerhofer PU, Tampé R.
Antigen Translocation Machineries in Adaptive Immunity and Viral Immune Evasion.
J Mol Biol. 2015 Mar 13;427(5):1102-1118. doi: 10.1016/j.jmb.2014.09.006. Epub 2014 Sep 16.

Thiele F, Tao S, Zhang Y, Muschaweckh A, Zollmann T, Protzer U, Abele R, Drexler I.
Modified Vaccinia Virus Ankara-Infected Dendritic Cells Present CD4+ T-Cell Epitopes by Endogenous Major Histocompatibility Complex Class II Presentation Pathways.
J Virol. 2015 Mar 1;89(5):2698-709. doi: 10.1128/JVI.03244-14. Epub 2014 Dec 17.

Fischbach H, Döring M, Nikles D, Lehnert E, Baldauf C, Kalinke U, Tampé R.
Ultrasensitive quantification of TAP-dependent antigen compartmentalization in scarce primary immune cell subsets.
Nat Commun. 2015 Feb 6;6:6199. doi: 10.1038/ncomms7199.

Bechara C, Nöll A, Morgner N, Degiacomi MT, Tampé R*, Robinson CV*.
A subset of annular lipids is linked to the flippase
activity of an ABC transporter.

Nat Chem. 2015 Mar;7(3):255-62. doi: 10.1038/nchem.2172. Epub 2015 Feb 2.

News & Views
             Harvey SR, Wysocki VH.
             Mass spectrometry: Bound in flight
             Nat Chem. 2015

Zollmann T, Moiset G, Tumulka F, Tampé R, Poolman B, Abele R.
Single liposome analysis of peptide translocation by the ABC transporter TAPL.
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2046-51. doi: 10.1073/pnas.1418100112. Epub 2015 Feb 2.

Kim J, Wu S, Tomasiak TM, Mergel C, Winter MB, Stiller SB, Robles-Colmanares Y, Stroud RM*, Tampé R*, Craik CS*, Cheng Y*.
Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter.
Nature. 2015 Jan 15;517(7534):396-400. doi: 10.1038/nature13872. Epub 2014 Nov 2.